Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha and beta tubulin subunits. Tubulinbinding drugs kill cancerous cells by inhibiting. Overnight nocodazole treated hela cells stained with purified mouse monoclonal antibody against gtubulin clone 14c11 followed by rhodamine redx conjugated donkey antimouse igg and dapi. The tubulin families include alpha, beta, gamma, delta, epsilon, and zeta tubulins. To test the apoptotic, necroptotic, and authopagic protein targets implicated in human normal fibroblasts treated with 3ma, western blotting was performed.
I am interested in buying an actin or tubulin antibody for western blot loading control. After rinses in pbs, the sections were incubated in secondary antibody goat anti rabbit conjugated with alexa 594 1. Pericentrin and gammatubulin form a protein complex and are. Protein names tubulin alpha1b chain, alphatubulin 3, alphatubulin ubiquitous, tubulin alpha3 chain, tubulin alphaubiquitous chain, tubulin balpha1. Betatubulin n14 is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the amino terminus of human betatubulin. Tubulin iii, is encoded by the gene mapped to human chromosome 16q24. Mouse monoclonal alphatubulin antibody 8f11 stj96937. Confocal immunofluorescent analysis of hela cells using. Mouse monoclonal antialphatubulin antibody stj96937. This antibody reacts with human,mouse,rat, dog samples. Tubulin 2 nucleates microtubules and is downregulated in mouse early. Tubulinassociated protein with an essential role in. Replacement to abcam, santa cruz, sigma and cst antibody. Antibody is provided at a concentration of 1 mg ml.
Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. The tuj1 antibody reacts with betatubulin iii, an 50 55 kda structural protein that is a component of tubulin. Please recommend us one of your actin andor tubulin products, and add it to the nox2 quotation. Antialphatubulin tuba1a antibody monoclonal, dm1a mouse igg monoclonal antibody for alphatubulin detection. Betatubulin loading control antibody, mouse mab catalog number. The gene that encodes alpha tubulin is highly similar to the mouse and rat tuba1 gene. The mammalian gammatubulin complex contains homologues of. Tubulin iii neuronal antibody produced in mouse has been used in following studies. Tubulin is a highly conserved protein with a molecular weight of 50 kd. Tubulin is required for maintaining the microtubule network.
Rabbit anti human tubulin alpha 4a chain antibody recognizes tubulin alpha 4a chain, also known as tubulin h2alpha, tubulin alpha1 chain, and tubulin, alpha 1 testis specific. Briefly, cells were seeded in 2% fbs medium overnight. Cells were fixed with 4% pfa, permeabilzed with 1% triton x100 in pbs, blocked with 10% serum, and incubated with mouse antihuman betatubulin monoclonal antibody at 4. Purified antitubulingamma antibody antigammatubulin. Antibodyplus provides trial size antibody samples for antibody validation. Confocal immunofluorescent analysis of c2c12 cells using. It is aimed to help labome visitors find the most suited tubulin antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers. Human tubulin alpha1b chain has a predicted length of 451 residues and mw of 50 kda. This antibody has been used to analyze the cell and tissue distributions of glutamylated tubulin.
It is localized to the microtubule organizing centres. Distribution of glutamylated alpha and betatubulin in. Validated in wb, ihc, iccif and tested in mouse, rat, cat, human, pig, xenopus laevis, zebrafish. Human mcf7 cell lysates probed with rabbit antitubulin beta polyclonal antibody, unconjugated bs4511r at 1. Tubulin 2 nucleates microtubules and is downregulated in mouse. This antibody reacts with human, mouse, rat, porcine, amphibian, bovine, canine, chicken, hamster, invertebrate, parasite, plant, primate, protozoa. Microtubules function in many essential cellular processes, including mitosis. Gcp2 and gcp3 colocalize with gammatubulin at the centrosome, cosediment. In mouse brain extracts, gt335 reacts strongly with alphatubulin and, to a. You will also have the opportunity to ask us for more information about the product.
Tubulin hela cos7 200 140 100 60 50 40 30 western blot analysis of extracts from hela and cos7 cells using. All information needed about our company and the product will be automatically attached. It binds two moles of gtp, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alphachain. The tubulin ac lys40 antibody 611b1 from novus biologicals is a mouse monoclonal antibody to tubulin.
The cells were treated with 1 mm 3ma for 4 hours, and the cells were collected and lysed with the buffer 1% sds, 10 mmoll triscl ph 7. Tubulin, gamma complex associated protein 4 tubgcp4. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The mouse polyclonal ascites that recognized xgrip109 by western blot analysis were used to. This product has not yet been tested in other species. Tubulin antibody produced in mouse is suitable for immunocytochemistry, western blot, microarray, indirect elisa, immunoprecipitation. Samples were transferred to a membrane and probed with gamma tubulin polyclonal antibody as a primary antibody and an hrpconjugated antirabbit igg was used as a secondary antibody. Purified antitubulingamma antibody antitubulingamma. This antibody reacts with human,dog,mouse,rat samples. Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of tubulin, which is a dimer of two 55kda subunits. Tubulin gamma western blot antibody biocompare antibody. There are multiple alpha and beta tubulin genes, which are highly conserved among species.
Thetm alpha tubulin antibody, mab, mouse antibody genscript. Tubulin, gamma complex associated protein 5 tubgcp5. Consult the supplier page to verify the identity of the desired antibody target and learn more detailed product information, such as species reactivity, antibody features, and validated applications. Provide accurate, reproducible results with the thermo scientific tubulin.
Monoclonal antibody is produced by immunizing animals with fulllength chicken. A trimer consisting of the tubulinspecific chaperone d tbcd, regulatory gtpase arl2, and. The tm alpha tubulin antibody, mab, mouse reacts with mouse, human, hamster, monkey, and pig. From abcams oem supplier st johns laboratory, validated in wb, ip. Tested in western blot wb, immunofluorescence if, immunocytochemistry icc, immunohistochemistry ihc and immunoprecipitation ip applications. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. Contents 1 function 2 interactions 3 references 4 external links 5. This antibody reacts with human, mouse, nonhuman primate, rat samples. Followed by a conjugated secondary antibody bs0295ghrp at 1. A linescan through a wildtype centrosome revealed a clear reduction in.
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